Titlebook: Bioluminescence Methods and Protocols; Robert A. LaRossa Book 19981st edition Springer Science+Business Media New York 1998

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Methods in Molecular Biologyhttp://image.papertrans.cn/b/image/187776.jpg

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Cryopreservation and Reawakeningving microorganisms for more than 50 years. There are numerous variations of the method, and elaborate equipment is available to improve environmental control and to allow large numbers of samples to be processed.

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Improvements in the Application of Firefly Luciferase Assaysefly luciferase reaction, AMP, PP., CO., and oxyluciferin are typical products that accumulate, but the product that is most often and most easily determined is light. The photons of light are not accumulated in the measuring technique unless film or some electronic summation procedure is used in photon counting.

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Bioluminescent Assay of the Adenylate Energy Chargegy can be stored and recovered by alteration of the ratio of the components. The charge of the three-component adenylate system is given by the mole fraction of ATP plus 1/2 of the mole fraction of ADP. It is shown in the following expression:

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Promoter Probe Vectorsll. Furthermore, utthzatton of the five-gene . reporter system allows continuous monitoring of light production, An additional advantage of the . reporter is the sensitivity and large dynamic range of light detection equipment, which enables very weak to very strong transcripttonal acttvtty to be measured.

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Abspann – Auszug aus einem Reiseberichtene can be identified by mapping the transposon insertion site. However, not all genes encode an obvious phenotype or readily assayable product. By coupling transcription or translation to an easily assayed reporter gene, it is possible to monitor expression of any gene, even if its phenotype is not known a ..