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Titlebook: Bioluminescence Methods and Protocols; Robert A. LaRossa Book 19981st edition Springer Science+Business Media New York 1998

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期刊全称Bioluminescence Methods and Protocols
影响因子2023Robert A. LaRossa
视频video
学科分类Methods in Molecular Biology
图书封面Titlebook: Bioluminescence Methods and Protocols;  Robert A. LaRossa Book 19981st edition Springer Science+Business Media New York 1998
影响因子The biological emission of light has fascinated humankind for mill- nia; who is not enchanted by the flashing of fireflies during summer‘s e- nings? Their collection by children in the area surrounding Baltimore, Maryland in the middle of this century for the purpose of biochemical isolation and ch- acterization of light producing proteins and cofactors may constitute the i- tiation of serious, quantitative studies of this phenomenon. The biochemistry of light production is certainly well-understood today. In bacterial systems it is coupled to the reducing and energy states of the cell, whereas in eukaryotic systems it is dependent upon the availability of ATP. These differences are reflected in the unrelated substrate requirements and structures of eukaryotic and prokaryotic luciferases. These biochemical studies have been aided by the cloning of luciferase-encoding structural genes, the structural genes for cofactor generation, and genes encoding proteins that interact with these lig- producing enzymes. These genes also provide starting materials for a wide range of applications that are the subject of this volume. Many of these advances have earlier been catalogued in two volume
Pindex Book 19981st edition
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Das ‚,ynergetic ,ntrepreneurship ,odel‘ plates are recommended for bright samples where reflection into neighboring wells results in “crosstalk.” White plates are recommended for samples that are lower light emitters, since the reflective surface enhances detection. Opaque plates are also available with transparent bottoms. Samples in the
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Komplexität — das gezähmte Chaosnse has been selected as a useful indicator of biological stress (.–.) because this response is universally found in btological systems and is induced by sublethal levels of a wide variety of cellular insults.
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Visualization of Bioluminescenceplates are recommended for bright samples where reflection into neighboring wells results in “crosstalk.” White plates are recommended for samples that are lower light emitters, since the reflective surface enhances detection. Opaque plates are also available with transparent bottoms. Samples in the
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Microscopic Imagery of Mammalian Cells Expressing an Enhanced Green Fluorescent Protein Goneed noninvasively in livmg cells by either fluorescence microscopy, flow cytometry, or macroscopic imaging technrques. GFP has been used as a reporter in a wide range of species, including a number of different mammalian cell lines (.). Moreover, a variety of N-and C-terminal protein fusions with GFP
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Genotoxic Sensors allows for the detection of agents that interact with DNA without a long incubation period. Many tests use . as the reporter for measuring transcriptional activation of promoters that are induced by DNA damage (.–.). In addition, .-prophage inductton assays (.,.) measures additional effects of the
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