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Titlebook: Bioluminescence Methods and Protocols; Robert A. LaRossa Book 19981st edition Springer Science+Business Media New York 1998

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楼主: otitis-externa
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Komplexität von Entscheidungsproblemenve bloluminescenct activity. Although normally localized in peroxisomes, luciferase folds to the native state on expression in bacteria, in mammahan cells In culture (.), and in rabbit reticulocyte lysate (RRL) (.–.). RRL also efficiently facilitates the renaturation of thermally (.–.) or chemically
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Komplexität von IT-Architekturenwith carcinogeneity in humans (.). The activation of bacterial DNA repair systems (recently reviewed; .,.) can be used as a measure of mutagenic and genotoxic effects of various chemical as well as physical treatments. Many of the gene products that act to repair DNA, however, are difficult to assay
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Komplexität — das gezähmte Chaos (.). Several of the induced proteins function to reactivate or degrade denatured proteins, which are the signal initiating the response. The heat-shock response, therefore, functions to maintam the proteinacious component of the cell in an active form. Thus, by inducing heat-shock protein synthesis
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https://doi.org/10.1007/978-3-658-05670-4based on the detection of adenosine triphosphate (ATP) are particularly useful, since it is possible to detect the ATP present in fewer than 20 cells/mL using optimized reagents. These same reagents can also accurately determine the ATP from 2 × 10. cells/mL in an adjacent well or test tube with a c
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Bioluminescence Methods and Protocols978-1-59259-577-8Series ISSN 1064-3745 Series E-ISSN 1940-6029
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Komplexität und Entrepreneurshipene expression in cases where protein product quantification is problematic. Reporters, such as /gbgalactostdase, provide convenient chromogenic assays (.). More recently, bioluminescence has been developed as a monitormg system (.). Reporter gene systems may be located extrachromosomally or integrated into the chromosome.
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Komplexität von Entscheidungsproblemenving microorganisms for more than 50 years. There are numerous variations of the method, and elaborate equipment is available to improve environmental control and to allow large numbers of samples to be processed.
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