Titlebook: Bioluminescence Methods and Protocols; Robert A. LaRossa Book 19981st edition Springer Science+Business Media New York 1998

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Probing for Promoters with Luciferase-Transposonsene can be identified by mapping the transposon insertion site. However, not all genes encode an obvious phenotype or readily assayable product. By coupling transcription or translation to an easily assayed reporter gene, it is possible to monitor expression of any gene, even if its phenotype is not known a ..

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Komplexität und Entrepreneurshipefly luciferase reaction, AMP, PP., CO., and oxyluciferin are typical products that accumulate, but the product that is most often and most easily determined is light. The photons of light are not accumulated in the measuring technique unless film or some electronic summation procedure is used in photon counting.

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https://doi.org/10.1007/978-3-658-13173-9gy can be stored and recovered by alteration of the ratio of the components. The charge of the three-component adenylate system is given by the mole fraction of ATP plus 1/2 of the mole fraction of ADP. It is shown in the following expression:

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https://doi.org/10.1007/978-3-658-05670-4ompletely linear relationship between cell number and light output, provided that the cells maintain a relatively invariant ATP content. Similar methods are used for bacteria and eukaryotic cells, but this chapter is restricted to the consideration of eukaryotic cells. Luminescence measurement of ATP levels uses the following reaction:

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Komplexität von Entscheidungsproblemenlferase with guani-diniumHCl was thought to generate unfolded luciferase, which closely mimics nascent unfolded luciferase. However, recent results indicate that the folding pathway of chemically denatured luciferase is not identical to that followed by newly synthesized luciferase (.).

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