Titlebook: Apoptosis and Cancer; Methods and Protocol Hugo Barcenilla,David Diaz Book 2022 The Editor(s) (if applicable) and The Author(s), under excl

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pulmonary

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High-Throughput, Parallel Flow Cytometry Screening of Hundreds of Cell Surface Antigens Using Fluor of technologies that would facilitate feasible and relatively cheap profiling of such a number of cells with an antibody-based approach led us to the development of a high-throughput cytometry-based platform for surface profiling. We coupled the fluorescent cell barcoding with preexisting, commerci

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Mass Cytometry Assessment of Cell Phenotypes and Signaling States in Human Whole Blood, Extension of this technique to mass cytometry (cytometry by time-of-flight or CyTOF) allows many more cell phenotypes and signaling nodes to be interrogated in parallel. The use of fresh whole blood is ideal for capturing the in vivo signaling state of all leukocytes, including granulocytes. In thi

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Quantification of Neutrophils Undergoing NET Formation and Distinguishing Mechanisms of Neutrophil ey roles in the pathogenesis of cancer and autoimmune diseases through multiple mechanisms including the formation of neutrophil extracellular traps (NETs). Further research to expand the understanding of neutrophils’ role in health and diseases is limited by lack of techniques to quantify neutrophi

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Cell Cycle Analysis of ER Stress and Autophagy,tress sensor signaling proteins PERK (protein kinase R-like endoplasmic reticulum kinase), IRE1, and ATF6 which initiate protein refolding and elongation of the ER until ER homeostasis is returned. If the misfolding of proteins is increased, then ER stress is maintained, and microautophagy of the ER

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Proximity Ligation in Situ Assay to Monitor Autophagy-Related Protein Interactions and Autophagy incalization of these interactions. PLISA can be used to quantify autophagy flux and can as well be adapted to assess global autophagy (SQSTM1/P62-LC3B interaction) or specific autophagy, such as mitophagy (NIX-LC3B). Here, we describe a step-by-step method to monitor autophagy using PLISA in adherent