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Titlebook: Apoptosis and Cancer; Methods and Protocol Hugo Barcenilla,David Diaz Book 2022 The Editor(s) (if applicable) and The Author(s), under excl

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Elemente der Siebenten Hauptgruppe IIemploying a predefined form of active signaling without the release of soluble cytoplasmic contents to the external environment. Compared to apoptosis, necrosis is a nonspecific form of sudden cell death in response to an invasive external stimulus which in turn is devoid of active programmed intrac
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Ueber die Bedingungen des Gleichgewichts,easurement of >50 protein moieties on the surface and inside the cell. The power of CyTOF lies in the application of purpose-built panels of antibody probes that resolve features of key biological processes in a cell. Here, we describe this technology’s use to profile changes in the intrinsic apopto
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,Am Molekülgerüst orientierte Bindungssätze, of technologies that would facilitate feasible and relatively cheap profiling of such a number of cells with an antibody-based approach led us to the development of a high-throughput cytometry-based platform for surface profiling. We coupled the fluorescent cell barcoding with preexisting, commerci
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Bausteinorientierte Synthesestrategie, Extension of this technique to mass cytometry (cytometry by time-of-flight or CyTOF) allows many more cell phenotypes and signaling nodes to be interrogated in parallel. The use of fresh whole blood is ideal for capturing the in vivo signaling state of all leukocytes, including granulocytes. In thi
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Elemente der Technologischen Mechanikey roles in the pathogenesis of cancer and autoimmune diseases through multiple mechanisms including the formation of neutrophil extracellular traps (NETs). Further research to expand the understanding of neutrophils’ role in health and diseases is limited by lack of techniques to quantify neutrophi
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Elemente der Technologischen Mechanikt in electrophilic metabolites which in turn induce protein carbonylations. Identification of specific carbonylated proteins and sites in ferroptotic cells will be of great significance for understanding the mechanism and discovering potential biomarkers for this new cell death. The protocol describ
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