Titlebook: Apoptosis and Cancer; Methods and Protocol Hugo Barcenilla,David Diaz Book 2022 The Editor(s) (if applicable) and The Author(s), under excl

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Detoxification

https://doi.org/10.1007/978-1-0716-2553-8Live-Content Imaging; Cell Imaging; microfluidic flow cytometry (µFCM); cell lysates; epithelial cells s

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978-1-0716-2555-2The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines

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Proximity Ligation in Situ Assay to Monitor Autophagy-Related Protein Interactions and Autophagy incalization of these interactions. PLISA can be used to quantify autophagy flux and can as well be adapted to assess global autophagy (SQSTM1/P62-LC3B interaction) or specific autophagy, such as mitophagy (NIX-LC3B). Here, we describe a step-by-step method to monitor autophagy using PLISA in adherent cancer cells.

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Accurate Enumeration of Apoptotic Cancer Cells Using Flow Cytometry,apoptotic cells displaying a specific linage antigen (LAg) within a population of cells that remain unfragmented and retain the expression of the LAg. However, this approach has two major limitations. Firstly, apoptotic cells fragment into apoptotic bodies that later disintegrate. Secondly, apoptoti

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Quantitative Analysis of Apoptosis and Necrosis in Live Cells Using Flow Cytometry,employing a predefined form of active signaling without the release of soluble cytoplasmic contents to the external environment. Compared to apoptosis, necrosis is a nonspecific form of sudden cell death in response to an invasive external stimulus which in turn is devoid of active programmed intrac