Titlebook: Apoptosis and Cancer; Methods and Protocol Hugo Barcenilla,David Diaz Book 2022 The Editor(s) (if applicable) and The Author(s), under excl

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Cell Cycle Analysis of ER Stress and Autophagy, or specifically reticulophagy occurs. However, LC3B, PERK, protein misfolding, and changes in ER mass (reticulophagy) can also be measured in a cell cycle-dependent manner by flow cytometry and the use of antibodies, protein misfolding, and ER tracking fluorescent probes.

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Simultaneous Detection of Inflammasome Activation and Membrane Damage During Pyroptosis,ly detect two hallmarks of inflammasome-mediated pyroptosis. Using a fluorescently tagged inflammasome adaptor protein (ASC-Citrine) and membrane-impermeable nuclear dyes, we can track inflammasome formation and plasma membrane disruption over time in the same cell population.

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Ueber die Bedingungen des Gleichgewichts,tic (cell death) protein machinery at a single-cell level. We provide a comprehensive overview of a tailor-made set of cell survival/death antibodies, ideal staining conditions, and high-dimensional data analysis.

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Bausteinorientierte Synthesestrategie,s chapter, we provide a detailed protocol for performing CyTOF phosphoflow in human whole blood, using cytokines and other stimuli. Barcoding and combining of multiple samples and other techniques to reduce batch effects and provide optimal comparability between samples/stimulations are also described.

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