Titlebook: Apoptosis and Cancer; Methods and Protocol Hugo Barcenilla,David Diaz Book 2022 The Editor(s) (if applicable) and The Author(s), under excl

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Elemente der Technologischen Mechanikly detect two hallmarks of inflammasome-mediated pyroptosis. Using a fluorescently tagged inflammasome adaptor protein (ASC-Citrine) and membrane-impermeable nuclear dyes, we can track inflammasome formation and plasma membrane disruption over time in the same cell population.

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Apoptosis and Cancer978-1-0716-2553-8Series ISSN 1064-3745 Series E-ISSN 1940-6029

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https://doi.org/10.1007/978-3-662-40293-1calization of these interactions. PLISA can be used to quantify autophagy flux and can as well be adapted to assess global autophagy (SQSTM1/P62-LC3B interaction) or specific autophagy, such as mitophagy (NIX-LC3B). Here, we describe a step-by-step method to monitor autophagy using PLISA in adherent cancer cells.

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Methods in Molecular Biologyhttp://image.papertrans.cn/a/image/159003.jpg

OATH

https://doi.org/10.1007/978-3-662-30593-5 real-time functionality allows for temporal resolution of apoptotic and cell death responses during the test exposure and obviates the need for onerous sample preparation and time course protocols associated with other annexin V methods. Further, this technique is eminently accessible to a wide ran

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dermatomyositis

Small-Intestine

Elemente der Siebenten Hauptgruppe Iapoptotic cells displaying a specific linage antigen (LAg) within a population of cells that remain unfragmented and retain the expression of the LAg. However, this approach has two major limitations. Firstly, apoptotic cells fragment into apoptotic bodies that later disintegrate. Secondly, apoptoti

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Elemente der Siebenten Hauptgruppe IIDuring efferocytosis, phagocytes are recruited to the site/activated by “find me” signals released from apoptotic cells, precisely identify apoptotic cells by the recognition of “eat me” signals on the apoptotic cell surface, and engulf the apoptotic cells to prevent secondary necrosis and inflammat