Titlebook: Viral Metagenomics; Methods and Protocol Vitantonio Pantaleo,Michela Chiumenti Book 2018 Springer Science+Business Media, LLC 2018 deep seq

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Double-Stranded RNA-Enriched Preparations to Identify Viroids by Next-Generation Sequencing, herbaceous and woody plants. Here we describe a protocol to extract nucleic acids from citrus bark and enrich them in double-stranded RNAs. These preparations can be efficiently used for generating cDNA libraries that, after pair-end sequencing and bioinformatics analyses, allow efficient identific

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Viral Metagenomics Approaches for High-Resolution Screening of Multiplexed Arthropod and Plant Vira describes an accurate and efficient approach to (1) concentrate viral particles from arthropods and plants, (2) remove contaminating non-encapsidated nucleic acids, (3) extract and amplify both viral DNA and RNA, and (4) analyze high-throughput sequencing (HTS) data by bioinformatics. Using this ap

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Different Approaches to Discover Mycovirus Associated to Marine Organisms,ine animal .. We provide detailed protocols for fungal isolation, fungal growth, and total RNA extraction. Ribosomal RNA depletion, cDNA library synthesis and normalization, and sequencing runs on different platforms are part of the protocols that are generally outsourced and therefore are not descr

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Use of siRNAs for Diagnosis of Viruses Associated to Woody Plants in Nurseries and Stock Collectiontively, the phytosanitary status of the propagation material (both the rootstock and the variety) can have a profound effect on the lifetime and health of the new plantations. The fast evolution of sequencing techniques provides a new opportunity for metagenomics-based viral diagnostics. Viral deriv

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The Use of High-Throughput Sequencing for the Study and Diagnosis of Plant Viruses and Viroids in Pl and total RNA sequencing. The protocol was developed for virus and viroid detection using NGS sequencing and was based on the results of a comparison between different extraction methods followed by yield, RNA purity, and integrity assessment. Moreover, the advantage of an additional ribosomal (r)