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Titlebook: Viral Metagenomics; Methods and Protocol Vitantonio Pantaleo,Michela Chiumenti Book 2018 Springer Science+Business Media, LLC 2018 deep seq

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发表于 2025-3-21 19:51:43 | 显示全部楼层 |阅读模式
书目名称Viral Metagenomics
副标题Methods and Protocol
编辑Vitantonio Pantaleo,Michela Chiumenti
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Viral Metagenomics; Methods and Protocol Vitantonio Pantaleo,Michela Chiumenti Book 2018 Springer Science+Business Media, LLC 2018 deep seq
描述.This volume explores the use of viral metagenomics to diagnose known viruses for plant and food production, human and animal health, and identifying viral vectors like insects. The chapters in this book cover topics, such as sRNAs isolation from tissues of grapevines and woody plants, high-resolution screening of arthropod and plant viral communities, identifying new pathogens in fish, detecting viruses in mycorrhizal fungi and their orchid host, and insect virus discovery through metagenomic and cell culture-based approaches. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Authoritative and comprehensive, .Viral Metagenomics: Methods and Protocols. is a valuable resource for researchers and specialists who are interested in learning more about this evolving field..
出版日期Book 2018
关键词deep sequencing; Next Generation Sequencing; cDNA library preparation; bioinformatics; siRNAs
版次1
doihttps://doi.org/10.1007/978-1-4939-7683-6
isbn_softcover978-1-4939-9257-7
isbn_ebook978-1-4939-7683-6Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC 2018
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Methods in Molecular Biologyhttp://image.papertrans.cn/v/image/983218.jpg
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Double-Stranded RNA-Enriched Preparations to Identify Viroids by Next-Generation Sequencing, herbaceous and woody plants. Here we describe a protocol to extract nucleic acids from citrus bark and enrich them in double-stranded RNAs. These preparations can be efficiently used for generating cDNA libraries that, after pair-end sequencing and bioinformatics analyses, allow efficient identification of the viroids infecting the source plant.
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Vitantonio Pantaleo,Michela ChiumentiIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
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Marion Cornelissen,Astrid Gall,Antoinette van der Kuyl,Chris Wymant,François Blanquart,Christophe Frperature or to improve the product yield of an equilibrium reaction. The controllability of such a system is found to depend strongly on the operating conditions, such as reactor temperature and composition of distillate, and on the time during the run. In general, controlling the reactor temperatur
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