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Titlebook: Viral Metagenomics; Methods and Protocol Vitantonio Pantaleo,Michela Chiumenti Book 2018 Springer Science+Business Media, LLC 2018 deep seq

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High-Resolution Screening of Viral Communities and Identification of New Pathogens in Fish Using Neopment of next-generation sequencing has enabled the possibility to sequence the entire virome of a tissue sample. This has led to an enormous increase in discovery of new viruses. In this chapter, we describe a simple and rapid method for viral discovery in fish. The method is based on Illumina seq
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From Whole-Genome Shotgun Sequencing to Viral Community Profiling: The ViromeScan Tool,ics sequencing. It efficiently denoises samples from reads of other microorganisms. Users can adopt the same shotgun metagenomic sequencing data to fully characterize complex microbial ecosystems, including bacteria and viruses. Here we apply ViromeScan pipeline to some examples, thus illustrating t
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Shannon Entropy to Evaluate Substitution Rate Variation Among Viral Nucleotide Positions in Datasetcted cells, tissues, and host organisms. In this chapter we present details on the use of the Shannon entropy method to estimate the site-specific nucleotide relative variability of turnip crinkle virus, a positive (+) stranded RNA plant virus, in a large dataset of short RNAs of . L., a natural res
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Book 2018viral vectors like insects. The chapters in this book cover topics, such as sRNAs isolation from tissues of grapevines and woody plants, high-resolution screening of arthropod and plant viral communities, identifying new pathogens in fish, detecting viruses in mycorrhizal fungi and their orchid host
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1064-3745 ation advice from the experts.This volume explores the use of viral metagenomics to diagnose known viruses for plant and food production, human and animal health, and identifying viral vectors like insects. The chapters in this book cover topics, such as sRNAs isolation from tissues of grapevines an
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