Titlebook: siRNA and miRNA Gene Silencing; From Bench to Bedsid Mouldy Sioud Book 2009 Humana Press 2009 Gene and immunotherapy.Gene regulation.In viv

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Deciphering the Code of Innate Immunity Recognition of siRNAs,pplications of siRNAs is to decipher the mechanisms involved in siRNA recognition by the immune system and to identify strategies that can evade immune activation. In this respect, the replacement of only uridines with their 2′-modified counterparts such as 2′-.-methyl uridines abrogate immune recog

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Targeted Delivery of Antisense Oligonucleotides and siRNAs into Mammalian Cells,e phage libraries or naturally occurring peptides. Also, transcriptional targeting strategies and detailed protocols for the selection of cancer cell-binding peptide from random peptide phage libraries are described.

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In Vitro and In Vivo Gene Silencing by TransKingdom RNAi (tkRNAi),ffers several advantages and has significant implications. First, this method allows the establishment of a long-term stable gene silencing system in the laboratory against genes of interests in vitro and in vivo, and enables high-throughput functional genomics screening in mammalian systems. RNAi l

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Bacterial Delivery of siRNAs: A New Approach to Solid Tumor Therapy,target cells. Attenuated . have been employed recently as vectors to deliver silencing hairpin RNA (shRNA) expression plasmids into mammalian cells. This approach has achieved gene silencing in vitro and in vivo. The facultative anaerobic, invasive . have a natural tropism for solid tumors including

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The Therapeutic Potential of LNA-modified siRNAs: Reduction of Off-target Effects by Chemical Modifhese problems are similar to those associated with classic antisense approaches, such as lack of delivery to specific tissues (other than the liver) or tumors, while other problems are more specific for siRNA, such as stability of the RNA molecules in circulation, off-target effects, interference wi

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pSM155 and pSM30 Vectors for miRNA and shRNA Expression,rons. Like the original miRNA vectors, these pSM155 and pSM30 constructs can efficiently downregulate the expression of their target genes. Moreover, the expression of a coexpressed fluorescent marker, EGFP, is substantially improved by this new design. The new vectors can also be used to express na

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