Titlebook: Super-Resolution Microscopy; Methods and Protocol Holger Erfle Book 2017 Springer Science+Business Media LLC 2017 Stimulated Emission Deple

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Correlative Fluorescence Super-Resolution Localization Microscopy and Platinum Replica EM on Unroofniques and produces images where localization microscopy data can be used to highlight specific proteins of interest within the structural context of the platinum replica TEM image. This combined method is uniquely suited to investigate the nanometer-scale structural organization of the plasma membrane and its associated organelles and proteins.

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Fully Automated Targeted Confocal and Single-Molecule Localization Microscopy,orkflow. Thus, measuring biological nanometre-sized structures in a sufficient number to get statistical significance becomes feasible. For proof of principle HIV-1 assembly complexes in HeLa cells derived from transfection of replication deficient viral construct were imaged by a fully automated screen.

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Live-Cell STED Imaging with the HyPer2 Biosensor,g of intracellular macromolecular architecture with simultaneous extraction of functional information about cellular activities. Here, we describe a protocol for live-cell STED microscopy of the HyPer2 biosensor fused to cytoskeletal filaments.

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Book 2017topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Cutting-edge and comprehensive, .Super-Resolution Microscopy:. .Methods and Protocols .is a valuable resource for both established and novel researchers and users in this field..

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Super-Resolution Microscopy Techniques and Their Potential for Applications in Radiation Biophysics of selected optical microscopy methods reaching super-resolution beyond the Abbe limit. We will survey three different concepts in connection to biological applications in radiation research without making a claim to be complete.