Titlebook: Super-Resolution Microscopy; Methods and Protocol Holger Erfle Book 2017 Springer Science+Business Media LLC 2017 Stimulated Emission Deple

observatory

Visual-Acuity

Brain Slice Staining and Preparation for Three-Dimensional Super-Resolution Microscopy, stochastic optical reconstruction microscopy (STORM)—provide the highest precision for single-molecule localization currently available. However, localization microscopy has been largely limited to cell cultures due to the difficulties that arise in imaging thicker tissue sections. Sample fixation

六边形

Scintillations

门窗的侧柱

Measuring Nanometer Distances Between Fluorescent Labels Step-by-Step,or at biomaterial interfaces. We here provide a protocol for Single-molecule High-Resolution Imaging with Photobleaching (SHRImP) that can be used to obtain information about the conformation of large proteins or other macromolecules at the single-molecule level. This procedure requires site-specifi

慷慨不好

Correlative Single-Molecule Localization Microscopy and Confocal Microscopy,biological questions the ability to provide tissue and cellular context in addition to these high resolution data is eminently informative. Here, we describe a procedure to achieve this aim by correlatively imaging human cardiac tissue first at the nanoscale with direct stochastic optical reconstruc

institute

极大痛苦

macabre

Four-Channel Super-Resolution Imaging by 3-D Structured Illumination,croscopy (SIM), which increases resolution by a factor of two. We use adherent, fixed cells to identify the localization of adhesion proteins using immunofluorescence and fluorescent proteins. We discuss how labeling with the necessary brightness is achieved and how data has to be processed for colocalization analysis.

渗透