Titlebook: Super-Resolution Microscopy; Methods and Protocol Holger Erfle Book 2017 Springer Science+Business Media LLC 2017 Stimulated Emission Deple

稀释前

Managing the Introduction of Super-Resolution Microscopy into a Core Facility,eing studied. Structured illumination techniques will give users a set of tools that are close to their past experience and relatively simple and quick to learn. The present dyes can be used. Resolution approaching 100 nm XY can be achieved. In contrast, stochastic methods such as PALM/STORM typical

inferno

Live-Cell STED Imaging with the HyPer2 Biosensor,be utilized as STED fluorophores, but also genetically encoded biosensors. Fusing the biosensor with proteins of interest allows subdiffraction imaging of intracellular macromolecular architecture with simultaneous extraction of functional information about cellular activities. Here, we describe a p

GUISE

Paleontology

恩惠

STED Imaging of Golgi Dynamics with Cer-SiR: A Two-Component, Photostable, High-Density Lipid Probey localization probe. Lipids are ideal high-density localization probes, as they are >100 times more abundant than most membrane-bound proteins and simultaneously demark the boundaries of cellular organelles. Here, we describe Cer-SiR, a two-component, high-density lipid probe that is exceptionally

新义

结束

Correlative SIM-STORM Microscopy, fluorescence light microscopy into an invaluable tool. However, conventional light microscopy is diffraction limited, which restricts the lateral resolution to around 200 nm laterally and 600–800 nm axially. In 2014, the Nobel Prize in Chemistry was awarded to Eric Betzig, Stefan W. Hell, and Willi

逗留

Correlative Super-Resolution Fluorescence Imaging and Atomic Force Microscopy for the Characterizattems at the nanoscale. High resolution imaging can be performed with a handful of techniques, each of them revealing particular features of the sample. A more comprehensive picture of a biological system can be achieved by combining the information provided by complementary imaging methods. Specific

critique

Quantitative Single-Molecule Localization Microscopy (qSMLM) of Membrane Proteins Based on Kinetic bdiffraction spatial resolution, additional information is available from observing single fluorophores over time. This includes the characteristic photophysical phenomenon of “blinking” that is exhibited by single fluorescent proteins or fluorophores and follows well-defined kinetic laws. Analyzing

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