Titlebook: Structure and Function of Membrane Proteins; Ingeborg Schmidt-Krey,James C. Gumbart Book 2021 Springer Science+Business Media, LLC, part o

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Kanokporn Chattrakun,Katherine G. Schaefer,Lucas S. Chandler,Brendan P. Marsh,Gavin M. King

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William G. Glass,Jonathan W. Essex,Franca Fraternali,James Gebbie-Rayet,Irene Marzuoli,Marley L. Sam

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Expression and Purification of Human Mitochondrial Intramembrane Protease PARL, expressing clones and monitoring during the large-scale expression and purification steps. We discuss the production protocol with precise details for each step. The protocol yields 1 mg of pure PARL per liter of yeast culture.

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Helical Membrane Protein Crystallization in the New Era of Electron Cryo-Microscopy,rotocol detailing the preparation of helical crystals, with an emphasis on further cryo-EM analysis and structural determination of the sarco(endo)plasmic reticulum Ca.-ATPase in the presence of regulatory subunits such as phospholamban.

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Methods in Molecular Biologyhttp://image.papertrans.cn/t/image/880206.jpg

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https://doi.org/10.1007/978-1-0716-1394-8Over-expression; Phospholipid bilayers; Protein purification; Functional studies; Protein structure