Titlebook: Structure and Function of Membrane Proteins; Ingeborg Schmidt-Krey,James C. Gumbart Book 2021 Springer Science+Business Media, LLC, part o

不断的变动

终止

DEI

Continuous Constant pH Molecular Dynamics Simulations of Transmembrane Proteins,ilibria. Here we describe the membrane-enabled hybrid-solvent continuous constant pH molecular dynamics method for capturing atomic details of proton-coupled conformational dynamics of transmembrane proteins. Example protocols from our recent application studies of proton channels and ion/substrate transporters are discussed.

photophobia

NMR Spectroscopic Studies of Ion Channels in Lipid Bilayers: Sample Preparation Strategies Exemplifd for the recombinant production of stable-isotope labeled hVDAC1 in ., the isolation of hVDAC1 from inclusion bodies and the refolding into detergent micelles, as well as the reconstitution of hVDAC1 into lipids to form 2D crystals.

vascular

affinity

记忆

密切关系

chastise

overrule

,Biochemical Characterization of GPCR–G Protein Complex Formation,ence of agonist or antagonist, as well as the complex dissociation in the presence of GTP analogue. Methods used in the protocol are affinity purification and gel filtration, which are also commonly used in protein sample preparation for structural work. Therefore, the protocol can be easily adapted for large-scale sample preparation.