Titlebook: Stem Cell Renewal and Cell-Cell Communication; Methods and Protocol Kursad Turksen Book 2021Latest edition Springer Science+Business Media,

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,Ultrastructural Analysis of Cell–Cell Interactions in Drosophila Ovary,urate spatiotemporal regulation of cell–cell interaction is critical for the development of eggs. Ultrastructural analysis using electron microscopy (EM) permits the visualization of both cells and subcellular signaling structures with high resolution. Here we describe a method for the processing of

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TIRF Microscopy as a Tool to Determine Exosome Composition, DNA. EVs are efficient platforms for intercellular communication, especially during immune responses, but also in some pathological contexts, such as tumor cell growth. The precise assessment of EV content is relevant for the selection of specific vesicles with specialized biological activities, wh

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Examining Local Cell-to-Cell Signalling in the Kidney Using ATP Biosensing,purines, with ligands such as adenosine triphosphate (ATP). Altered cell-to-cell communication, and in particular changes in the paracrine release of extracellular ATP, plays crucial roles in pathophysiological conditions, such as diabetes. ATP biosensing provides a reliable, real-time measurement o

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Isolation and Assessment of Pancreatic Islets Versus Dispersed Beta Cells: A Straightforward Approach as beta cells (~70%), alpha cells (~20%), delta cells (~5%), F cells (~4%), and epsilon cells (1%), each with its own unique function. To better understand the roles of these cells and how cell communication alters their function, several techniques have been established such as islet isolation a

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Angiogenesis

Preserving Cytonemes for Immunocytochemistry of Cultured Adherent Cells,tandard fixation techniques to study protein localization and interactions. A recent methodological advance has yielded improvements in cytoneme preservation using glutaraldehyde fixation and sodium borohydride treatment to reduce background. We herein describe a safer method for effective blocking

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