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Titlebook: Stem Cell Renewal and Cell-Cell Communication; Methods and Protocol Kursad Turksen Book 2021Latest edition Springer Science+Business Media,

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Chemical and Voltage Gating of Gap Junction Channels Expressed in , Oocytes, communication provides a fundamental mechanism for coordinating many cellular functions in mature and developing organs, as it enables free exchange of small cytosolic molecules. Gap junction channels are regulated by a chemical gating mechanism sensitive to cytosolic calcium concentration [Ca.]. i
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Analysis of Epithelial Architecture and Planar Spindle Orientation in the Drosophila Wing Disc,tic spindle in the wing disc epithelium. The rapid dissection method, effective immunostaining, and mounting tips described here facilitate genetic and cell biological studies of the wing disc and can be applied to a wide array of studies using various . tissues.
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Isolation and Assessment of Pancreatic Islets Versus Dispersed Beta Cells: A Straightforward Approand beta cell dispersion. Here we describe how to isolate primary rodent islets, disperse pancreatic islets, measure intracellular calcium, and use immunofluorescent staining to distinguish beta cells and alpha cells.
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Fluorescent Labeling of Connexin with As Complex and X-Y Coordinate Registration of Target Single C this tetra-Cys (TC) tag to form a fluorescent conjugate. Its introduction into the C-terminal of Cx43 is demonstrated. Next, a novel triangle chip for the accurate x-y registration is described. Target single cells of HeLa marked with a fluorescent dye can be easily recognized by electron microscopy based on this chip.
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Book 2021Latest editionablished laboratories around the world. The contents represent the great strides made in the field of cell-cell communications with respect to the identification and characterization of key components of the communication apparatus, assembly and maintenance of the communications structures, and conc
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