Titlebook: Stem Cell Renewal and Cell-Cell Communication; Methods and Protocol Kursad Turksen Book 2021Latest edition Springer Science+Business Media,

Condense

Chemical and Voltage Gating of Gap Junction Channels Expressed in , Oocytes, is the double voltage clamp of . oocytes. Basically, this method involves pairing at the vegetal pole devitellinized oocytes in a conical well of a culture dish, inserting in each of them a current and a voltage microelectrode, establishing double voltage clamp and measuring junctional conductance (.j) from voltage and current records.

TRACE

,Inference of Ligand–Receptor Pairs from Single-Cell Transcriptomics Data,ith a computational approach to identify cell-type specific and biologically relevant interactions. Here, we summarize the structure and content of our repository and present a practical guide for inferring cell–cell communication networks from single-cell RNA sequencing data.

Frenetic

Purification of the , Quorum-Sensing Transcription Factors LuxR, HapR, and SmcR,t are pure, active, and concentrated to perform various assays. The methods described here are optimized for purification of the . master quorum-sensing regulators, LuxR (.), HapR (.), and SmcR (.). We anticipate that these methods can be applied to other proteins in this family of transcription factors.

虚度

Emg827

Multiple Imaging Modalities for Cell-Cell Communication via Calcium Mobilizations in Corneal Epithemonly used fluorescent indicators derived from the calcium chelator BAPTA. Here we describe sample protocols that detail how these indicators are used in in vitro and ex vivo experiments to analyze the role of calcium mobilizations in cell-cell communication and coordinated cellular motility in the

眼界

烤架

披肩

PET-scan

A Co-culture Model to Study the Effect of Kidney Fibroblast-p90RSK on Epithelial Cell Survival,al cells. However, in recent years, growing numbers of studies have been shifting toward the role of the intercellular interactions, such as communication between tubular epithelial cells and fibroblasts. Various co-culture models have been utilized in the studies of cell-cell communication and inte

Hemodialysis

Calcium Fluorescence Recordings from Neuroepithelial Stem Cells,ntact with the basement membrane of the neuroepithelium. Under such circumstances, Ca. fluorescence recording is a successful method to study physiological properties of the neuroepithelial stem cell. This chapter describes detailed techniques of Ca. fluorescence recording from neuroepithelial stem