Titlebook: Stem Cell Assays; Methods and Protocol Nagarajan Kannan,Philip Beer Book 2022 Springer Science+Business Media, LLC, part of Springer Nature

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Culture of Human iPSC-Derived Motoneurons in Compartmentalized Microfluidic Devices and Quantitativef microfluidic chips and programs designed for scientific multidimensional imaging. We have used this approach to analyze axonal phenotypes. These tools are useful to investigate the cellular and molecular bases of neuromuscular diseases, including amyotrophic lateral sclerosis and spinal muscular atrophy.

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Rapid and Highly Efficient Isolation and Purification of Human Induced Pluripotent Stem Cellsany challenges. Here we describe a simple yet highly efficient two-step protocol for the isolation and purification of human iPSC lines. The first step adapts iPSCs to single cell culture and passaging, promoting survival and self-renewal; the second step enables the isolation and purification of bo

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Artificial Activation of Murine Oocytes Using Strontium to Derive Haploid and Diploid Parthenotesion from spermatozoon. This phenomenon does not occur naturally in placental mammals. However, the mammalian oocytes can be artificially activated in vitro using mechanical, electrical, and chemical stimuli which can develop up to the blastocyst stage. In this chapter, we describe the protocol for g

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Generation of Human iPSC from Small Volume Peripheral Blood Samplest. In particular, the generation of iPSCs from patients who suffer from inherited diseases is of great interest, as most of these diseases are rare and not well studied. As most affected patients are diagnosed during infancy, the derivation of somatic cells for the generation of iPSCs is very much l

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Titlebook: Stem Cell Assays; Methods and Protocol Nagarajan Kannan,Philip Beer Book 2022 Springer Science+Business Media, LLC, part of Springer Nature

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