Titlebook: Stem Cell Assays; Methods and Protocol Nagarajan Kannan,Philip Beer Book 2022 Springer Science+Business Media, LLC, part of Springer Nature

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Distinguishing Between Endodermal and Pluripotent Stem Cell Lines During Somatic Cell Reprogrammingnic endoderm stem (iXEN) cells. Since both stem cells types routinely arise alongside one another during reprogramming, it is critical to distinguish between both cell types to ensure that the desired cell population is selected and analyzed. This chapter details, from start to finish, how to reprog

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Measuring Early Germ-Layer Specification Bias in Human Pluripotent Stem Cellshere is significant variation in the potential of individual cell lines to differentiate towards each of the three germ layers as a result of (epi)genetic background, culture conditions, and other factors. We describe here in detail a methodology to evaluate this bias using short directed differenti

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Detection of Soluble and Insoluble Protein Species in Patient-Derived iPSCstive disease, methods of detection vary from one model system to another. Induced pluripotent stem cells (iPSCs) present an opportunity to model disease using patient-specific cells. However, iPSC-derived neurons are fetal-like in maturity, making it a challenge to detect key features such as protei

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Assessment of Endothelial-to-Hematopoietic Transition of Individual Hemogenic Endothelium and Bulk Pn-adherent blood cell morphology. Investigating the mechanisms of EHT is critical for understanding the development of hematopoietic stem cells (HSCs) and the entirety of the adult immune system, and advancing technologies for manufacturing blood cells from human pluripotent stem cells (hPSCs). Here

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iPS Cell Differentiation into Brain Microvascular Endothelial Cellson to the brain microenvironment from toxins or any fluctuations in the composition of the blood plasma via a layer of endothelial cells connected by tight junctions and supported by pericytes and astrocytes. Disruption of the barrier can be either a cause or a consequence of central nervous system