Titlebook: Spliceosomal Pre-mRNA Splicing; Methods and Protocol Klemens J. Hertel Book 2014 Springer Science+Business Media, LLC 2014 Alternative spli

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Maliheh Movassat,William F. Mueller,Klemens J. Hertel

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Diversity and Evolution of Spliceosomal Systemsdiverse eukaryotic species now allows for the reconstruction of the evolutionary history of this diversity. Consideration of spliceosomal systems in comparative context reveals a surprising and very complex portrait: in contrast to many expectations, gene structures in early eukaryotic ancestors wer

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Alternative Pre-mRNA Splicingve splicing, as well as the functional role, highlighting how alternative splicing varies across phylogeny. Regulated alternative splicing can affect protein function and ultimately impact biological outcomes. We examine the possibility that portions of alternatively spliced transcripts are the resu

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Regulation of Alternative Pre-mRNA Splicingding genes. Alternative splicing is frequently regulated with cell type or developmental specificity and in response to signaling pathways, and its mis-regulation can lead to disease. Co-regulated programs of alternative splicing involve interplay between a host of .-acting transcript features and .

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Preparation of Splicing Competent Nuclear Extracts increase the difficulty of studying the splicing reaction in detail. Nuclear extracts derived from HeLa cells provide all of the obligatory components to carry out intron removal in vitro. This chapter describes the large-scale preparation of nuclear extract from HeLa cells.

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