Titlebook: Spliceosomal Pre-mRNA Splicing; Methods and Protocol Klemens J. Hertel Book 2014 Springer Science+Business Media, LLC 2014 Alternative spli

影响

RLS898

The Pre-mRNA Splicing Reactiong removes introns and joins exons to form mature mRNAs. It is catalyzed by a large RNP complex called the spliceosome. Sequences within the pre-mRNA determine intron recognition and excision. This process occurs with a high degree of accuracy to generate the functional transcriptome of a cell.

infarct

Preparation of Splicing Competent Nuclear Extracts increase the difficulty of studying the splicing reaction in detail. Nuclear extracts derived from HeLa cells provide all of the obligatory components to carry out intron removal in vitro. This chapter describes the large-scale preparation of nuclear extract from HeLa cells.

建筑师

Kinetic Analysis of In Vitro Pre-mRNA Splicing in HeLa Nuclear Extractions from functional elements for the efficient removal of introns. This chapter will describe the rationale and approach employed to use kinetic analysis to evaluate an in vitro splicing reaction using radiolabeled pre-mRNA incubated in splicing-competent HeLa nuclear extract (NE).

描述

HAWK

https://doi.org/10.1007/978-1-62703-980-2Alternative splicing; Functional mRNAs; Multiple protein isoforms; Nuclear pre-mRNAs; Protein coding seq

Corporeal

exophthalmos

Panther

A Conceptual and Effective Scheme for Brain Tumor Identification Using Robust Random Forest Classifierin tumor identification experiments carried out using MATLAB and evaluates the better performance. The outcome of the results compared with the other conventional brain tumor identification techniques.

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