书目名称 | Spliceosomal Pre-mRNA Splicing |
副标题 | Methods and Protocol |
编辑 | Klemens J. Hertel |
视频video | |
概述 | Introduces protocols and their theoretical background relevant for a variety of experimental research in pre-mRNA splicing.Provides step-by-step detail essential for reproducible results.Contains key |
丛书名称 | Methods in Molecular Biology |
图书封面 |  |
描述 | .Providing a guide to classical experimental approaches to decipher splicing mechanisms and experimental strategies that rely on novel multi-disciplinary approaches, .Spliceosomal Pre-mRNA Splicing: Methods and Protocols. describes the theory of alternative pre-mRNA splicing in seven introductory chapters and then introduces protocols and their theoretical background relevant for a variety of experimental research. These protocol chapters cover basic methods to detect splicing events, analyses of alternative pre-mRNA splicing .in vitro. and .in vivo. manipulation of splicing events and high-throughput and bioinformatic analyses of alternative splicing. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and tips on troubleshooting and avoiding known pitfalls..Comprehensive and practical, .Spliceosomal Pre-mRNA Splicing: Methods and Protocols. will aid newcomers and seasoned molecular biologists in understanding the fascinating world of alternative splicing with the ultimate goal of paving the way for many n |
出版日期 | Book 2014 |
关键词 | Alternative splicing; Functional mRNAs; Multiple protein isoforms; Nuclear pre-mRNAs; Protein coding seq |
版次 | 1 |
doi | https://doi.org/10.1007/978-1-62703-980-2 |
isbn_softcover | 978-1-4939-6044-6 |
isbn_ebook | 978-1-62703-980-2Series ISSN 1064-3745 Series E-ISSN 1940-6029 |
issn_series | 1064-3745 |
copyright | Springer Science+Business Media, LLC 2014 |