Titlebook: Small Non-Coding RNAs; Methods and Protocol Mathieu Rederstorff Book 2021Latest edition Springer Science+Business Media, LLC, part of Sprin

granite

Purification of RiboNucleoProtein Particles by MS2-MBP Affinity Chromatographying of three stem-loops that provide specific binding sites for the phage MS2 protein. Here, we successfully applied this method to isolate RNPs formed with subfragments of the long noncoding RNA ANRIL (Antisense Noncoding RNA in the INK4 Locus).

overreach

胖人手艺好

MAZE

MERIT

RNA Precipitation, basic protocol with key advices to observe, but numerous variations on the theme are discussed. Indeed, several important parameters, such as the choice of salt, alcohol, or carrier, have to be considered to improve the efficiency of precipitation and the yield of RNA recovery.

是剥皮

Resistance

委托

Northern Blot Detection of Tiny RNAsd immobilization on membranes as well as design of sensitive detection probes. For RNA crosslinking to positively charged membranes, we compared UV light with chemical RNA crosslinking by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), using either denaturing or native polyacrylamide gels. We

NAIVE

Stem-Loop qRT-PCR–Based Quantification of miRNAsod is divided in two steps. First, RNA is reverse transcribed using a specific stem-loop primer, and the resulting RT product is subsequently used as a template for quantitative real-time PCR. This fast and simple method provides quantitative data with high sensitivity and specificity to study miRNA

peak-flow

Detection and Labeling of Small Non-Coding RNAs by Splinted Ligationxpression patterns. The splinted ligation method described here is based on nucleic acid hybridization. It is optimized for the direct labeling and quantitative detection of small RNAs. A specific bridge DNA oligonucleotide is used, which is perfectly complementary to both the target small RNA and a