Titlebook: Small Non-Coding RNAs; Methods and Protocol Mathieu Rederstorff Book 2021Latest edition Springer Science+Business Media, LLC, part of Sprin

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书目名称Small Non-Coding RNAs影响因子(影响力)学科排名




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书目名称Small Non-Coding RNAs被引频次学科排名




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书目名称Small Non-Coding RNAs读者反馈




书目名称Small Non-Coding RNAs读者反馈学科排名

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FACET

Microarray Analysis of Whole-Transcriptome RNAs Including Non-Coding RNAss (no limitation by read depth). This chapter describes the protocols used in our lab to extract and purify total RNAs from PAXgene RNA Blood Tubes and to perform RNA labeling and hybridization on the Clariom™ D microarrays from Affymetrix.

perpetual

Northern Blot Detection of Tiny RNAs hands, more sensitive than using a corresponding DNA/LNA mixmer probe with a 5′-.P-end-label. Furthermore, we provide a robust protocol for northern blot analysis of noncoding RNAs of intermediate size (~50–400 nt).

小平面

Using Native RIP, UV-CLIP or fCLIP to Address Protein–RNA Interactions In Vivofy RNA interactions, while crosslinked RIP (CLIP) may inform about direct interactions as well as their extent in the unaltered cellular condition, i.e., before cell lysis. In this chapter, both techniques applied to mammalian cells are described with a series of precautions regarding their design.

不能仁慈

Study of Genome-Wide Occupancy of Long Non-Coding RNAs Using Chromatin Isolation by RNA Purificationhe genomic binding sites of several nuclear lncRNAs. In this chapter, we describe the ChIRP protocol that was successfully applied to the lncRNA ANRIL. We also provide a user-friendly bioinformatic pipeline for ChIRP-seq data analysis.

CHAFE

CRISPR/Cas9 System to Knockdown MicroRNA In Vitro and In Vivohat CRISPR/Cas9 is an economic, convenient, and innovative strategy with high efficiency, specificity, and stability for the modulation of miRNA expression. Herein, we describe a detailed protocol for knocking out miRNA genes in vitro and in vivo with the CRISPR/Cas9 system.

tympanometry

1064-3745 ation advice from the experts.This second edition provides new and updated chapters detailing methods tackling all aspects of small non-coding RNAs biology.  Chapters guide readers through customized dedicated protocols and technologies that will be of valuable help decipher the numerous functions o

Assemble

Non-Coding RNA Silencing in Mammalian Cells by Antisense LNA GapmeRs Transfectionthe silencing of two different nuclear ncRNAs (ANRIL and SATIII RNAs) in mammalian cells using antisense LNA GapmeRs with two different transfection methods: calcium phosphate-mediated transfection and Lipofectamine. 2000.

Mundane

Detection and Labeling of Small Non-Coding RNAs by Splinted Ligation labeled ligation nucleic acid. The target RNA is subsequently labeled by ligation, detected by analysis in denaturing conditions, and quantified by phosphorimaging. The protocol does not require any specific material, and the procedure is fast and sensitive.