Titlebook: Small Non-Coding RNAs; Methods and Protocol Mathieu Rederstorff Book 2021Latest edition Springer Science+Business Media, LLC, part of Sprin

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Fluorescence In Situ Hybridization of Small Non-Coding RNAs detect specific RNAs in fixed cells. It allows both localization and quantification of RNA molecules within individual cells and tissues. Refined RNA-FISH methods have also been developed to determine RNA transcription and degradation rates. This chapter describes an RNA-FISH protocol that we devel

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Using Native RIP, UV-CLIP or fCLIP to Address Protein–RNA Interactions In Vivos to modulate their fate and promote their activity. The identification of such interactions as well as the cellular and molecular conditions of these interactions represent key information for the characterization of the role of each partner. RNA immunoprecipitation (RIP) is the leading technique t

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CRISPR/Cas9 System to Knockdown MicroRNA In Vitro and In Vivotion of gene expression at the post-transcriptional and translational levels. Loss-of-function studies are the fundamental strategy to examine miRNA function and target genes in cellular and molecular biology. Traditional methods for miRNA loss-of-function studies include miRNA-specific antisense in

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Microarray Analysis of Whole-Transcriptome RNAs Including Non-Coding RNAsls for therapy, ncRNAs have been extensively detected in body fluids supporting their role as easily accessible and minimally invasive biomarkers. However, the precise measurement of circulating ncRNAs remains challenging due to their low abundance and the heterogeneity of the ncRNA population (size

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Isolation, Extraction and Deep-Sequencing Analysis of Extracellular RNAs (exRNAs) from Human Plasmalable sample for biomedical analysis, was reported to contain various subpopulations of exRNA, some of which are most likely components of plasma ribonucleoproteins (RNPs), while others are encapsulated into extracellular vesicles (EVs) of different size, origin, and composition. Unbiased analysis o

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