Titlebook: Small Non-Coding RNAs; Methods and Protocol Mathieu Rederstorff Book 2015 Springer Science+Business Media New York 2015 Biogenesis.Gene Exp

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Quantification and Quality Control of a Small Non-Coding RNA Preparatione in gene regulation and diseases. RNA quantification and control of RNA integrity are two key steps in sncRNA profiling. In this chapter, we will describe different gold standard methods used to achieve both purposes before the use of the RNAs in downstream applications.

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Impact of RNA Isolation Protocols on RNA Detection by Northern Blottingtection of tiny RNAs (~14-mers) or RNAs of intermediate size (100–200 nt) in terms of signal quality, intensity, and reproducibility. Our analysis included two hot phenol methods and two TRIzol extraction procedures. We found that signal intensity/detection sensitivity makes the key difference. Tota

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RT-qPCR-Based Quantification of Small Non-Coding RNAsn gene expression regulation, and their involvement in disease biology is well documented. This has fueled the development of numerous tools for the quantification of miRNA expression levels. These tools are based on three technologies: (microarray) probe hybridization, RNA sequencing, and reverse t