Titlebook: Small Non-Coding RNAs; Methods and Protocol Mathieu Rederstorff Book 2015 Springer Science+Business Media New York 2015 Biogenesis.Gene Exp

GAVEL

nepotism

entice

Direct Cloning of Double-Stranded RNAsMost annotated genomes show a large number of sense–antisense transcripts that can generate double-stranded RNAs. We describe a method to clone these dsRNAs from total RNA preparations.

Acetaldehyde

表被动

978-1-4939-4903-8Springer Science+Business Media New York 2015

抱怨

Small Non-Coding RNAs978-1-4939-2547-6Series ISSN 1064-3745 Series E-ISSN 1940-6029

CRATE

Multiple

Alcoholic Precipitation of Small Non-Coding RNAsa standard, basic protocol with key advices to observe, but numerous variations on the theme are discussed. Indeed, several important parameters, such as the choice of salt, alcohol, or carrier, have to be considered to improve the efficiency of precipitation and the yield of RNA recovery.

Abrupt

丑恶

Stem-Loop RT-PCR Based Quantification of Small Non-Coding RNAsod is divided in two steps. First, RNA is reverse-transcribed using a specific stem-loop primer, and the resulting RT product is subsequently used as a template for quantitative real-time PCR. This fast and simple method provides quantitative data with high sensitivity and specificity to study miRNAs and their functions.