Titlebook: Small Non-Coding RNAs; Methods and Protocol Mathieu Rederstorff Book 2015 Springer Science+Business Media New York 2015 Biogenesis.Gene Exp

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1064-3745 Protocols. reaches out to biochemists, cellular and molecular biologists already working in the field of RNA biology  and to those just starting to study small non-coding RNAs..978-1-4939-4903-8978-1-4939-2547-6Series ISSN 1064-3745 Series E-ISSN 1940-6029

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Improved Northern Blot Detection of Small RNAs Using EDC Crosslinking and DNA/LNA Probesrn blot detection of tiny RNAs with 5′-digoxigenin-labeled DNA/LNA mixmer probes is a highly sensitive and specific method and, in our hands, more sensitive than using a corresponding DNA/LNA mixmer probe with a 5′-.P-end label.

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Fluorescence In Situ Hybridization of Small Non-Coding RNAss expressed in response to various cellular stresses including heat shock. This protocol is based on the use of a biotinylated LNA probe subsequently detected by a streptavidin–Alexa Fluor. 488 conjugate. A protocol allowing efficient coupling of RNA FISH and protein detection by immunofluorescence is also described in this chapter.

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Microarray Analysis of Small Non-Coding RNAsws sensitive profiling of small ncRNAs regardless of their sequence. Here, we describe the isolation and labeling of small non-coding RNAs, as well as their hybridization to microarrays with LNA-modified oligonucleotide probes using a semi-automated hybridization device.

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Impact of RNA Isolation Protocols on RNA Detection by Northern Blottingarations which instead enrich for RNAs of tRNA size and smaller. Thus, hot phenol methods are the choice for the detection of intermediate-sized and longer RNAs, whereas TRIzol extraction procedures are more suited for the detection of tiny RNAs.

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