Titlebook: Skin Stem Cells; Methods and Protocol Kursad Turksen Book 2019Latest edition Springer Science+Business Media, LLC, part of Springer Nature

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Decellularized bSIS-ECM as a Regenerative Biomaterial for Skin Wound Repair,tential for overcoming these limitations by supporting the regenerative activity and localization of stem cells. This chapter describes the use of bone marrow mesenchymal stem cells together with decellularized bovine small intestinal submucosa (SIS), for the treatment of a critical-sized full-thickness skin defect in a small animal model.

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1064-3745 essential for reproducible results.Contains key notes and imThis detailed book collects new and updated techniques that will help to improve our understanding of the mechanisms underlying stem cell-derived repair through stem cells of the epidermal and dermal lineages, which have led to the isolatio

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Book 2019Latest editionitfalls. .Comprehensive and practical, .Skin Stem Cells: Methods and Protocols, Second Edition. serves as a valuable assemblage of protocols for researchers both already working in the field and those who now wish to newly embark on studies of skin stem cells..

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Ex Vivo Imaging and Genetic Manipulation of Mouse Hair Follicle Bulge Stem Cells,rimental pipeline to complement in vivo studies. Here we describe detailed methods for culturing, immunostaining, live cell imaging, and adenoviral infection of bulge stem cells for downstream applications such as in vitro clonal and in vivo patch assays.

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Magnetic-Based Cell Isolation Technique for the Selection of Stem Cells,be the isolation of stem cells by MACS using CD34 antigen for SkSCs, again CD34 for hematopoietic stem cells (HSCs) and Thy-1 for spermatogonial stem cells (SpSCs). MACS allowed the isolation of CD34+, CD34+, and Thy-1+ human SkSCs, HSCs, and SpSCs with minimum 98% purity.