Titlebook: Skin Stem Cells; Methods and Protocol Kursad Turksen Book 2019Latest edition Springer Science+Business Media, LLC, part of Springer Nature

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Quantitative Detection of Low-Abundance Transcripts at Single-Cell Level in Human Epidermal Keratinus to determine the number of RNA molecules in a given cell sample. Here, we describe a workflow that we have set up to meet these specific needs, by means of transcript quantification in cellular micro-samples using flow cytometry sorting and reverse transcription-digital droplet polymerase chain r

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Qualitatively Monitoring Binding and Expression of the Transcription Factors Sp1 and NFI as a Usefueen reconstructed tissues exhibiting poor histological and structural characteristics and a low expression of Sp1 in their constituting epithelial cells. Therefore, monitoring both the expression and DNA binding of these transcription factors in human skin and corneal epithelial cells is a useful to

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1064-3745 tion. serves as a valuable assemblage of protocols for researchers both already working in the field and those who now wish to newly embark on studies of skin stem cells..978-1-4939-8870-9Series ISSN 1064-3745 Series E-ISSN 1940-6029

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Gaëtan Le-Bel,Sergio Cortez Ghio,Danielle Larouche,Lucie Germain,Sylvain L. Guérin

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