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Titlebook: Skin Stem Cells; Methods and Protocol Kursad Turksen Book 2019Latest edition Springer Science+Business Media, LLC, part of Springer Nature

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Whole-Mount Immunofluorescent Staining Coupled to Multicolor Lineage Tracing Model for Analyzing thogenitors. We introduce a whole-mount immunofluorescent staining coupled to a multicolor lineage tracing model for analyzing the spatiotemporal organization of epidermal stem cells. Laser scanning confocal microscopy with multiple labelling allows for robust imaging of dorsal skin with excellent res
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Isolation and Enrichment of Newborn and Adult Skin Stem Cells of the Interfollicular Epidermis,hereby balancing cell loss in tissue turnover or in wound repair. In this chapter, we describe a reliable and simple method for isolating interfollicular epithelial stem cells from the skin of newborn mice or from tail and ear skin of adult mice using fluorescence-activated cell sorting (FACS). We a
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One-Step Simple Isolation Method to Obtain Both Epidermal and Dermal Stem Cells from Human Skin Spey and quality of skin stem cells is important for both laboratory studies and clinical applications. Here, we describe a one-step isolation procedure to efficiently obtain both epidermal and dermal cell population from human skin specimen, based on the different influence of the Rho kinase inhibitor
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Magnetic-Based Cell Isolation Technique for the Selection of Stem Cells,ue enables the isolation and selection of germ, hematopoietic, and somatic stem cells including skin stem cells (SkSCs). Here, we have tried to describe the isolation of stem cells by MACS using CD34 antigen for SkSCs, again CD34 for hematopoietic stem cells (HSCs) and Thy-1 for spermatogonial stem
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Isolation of Human Skin Epidermal Stem Cells Based on the Expression of Endothelial Protein C Recepmis. IESCs also contribute to the repair and regeneration of the epidermis during wound healing. The great plasticity and easy accessibility afforded by IESCs make them a promising source of stem cells for scientific research and clinical applications. Thus, simple methods to isolate and define pure
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Protocols for Full Thickness Skin Wound Repair Using Prevascularized Human Mesenchymal Stem Cell Sh not viable, such as in the case of wounds with large surface areas. The donor sites of STSGs may be harvested repeatedly, but STSG transplants are still limited by insufficient blood supply at the early stages of wound healing. Prevascularized human mesenchymal stem cell (hMSC) sheets may accelerat
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