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Titlebook: Skeletal Development and Repair; Methods and Protocol Matthew J. Hilton Book 2014 Springer Science+Business Media, LLC 2014 cell assays.ske

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Beta-Galactosidase Staining in the Skeletonle-mount or on tissue sections. In this protocol we provide detailed methods for visualizing this reporter gene for both in vivo and in vitro studies. In addition, we describe a few tips for troubleshooting for mineralized tissues such as the skeleton.
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Radioactive In Situ Hybridization to Detect Gene Expression in Skeletal Tissue Sectionsspatial information about gene expression. Compared to the nonradioactive alternatives,.S radioactive ISH generally provides higher sensitivity. Here, we describe the procedure for.S ISH on paraffin sections from the skeletal tissues of mouse embryos.
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Book 2014s the platform for surgical manipulation and/or transplantation as well as the source of tissues and cells for .in vitro .culture and analyses. Chapters are written by experts in the field and cover topics including surgical, transplantation and organ culture methods that permit analyses of skeletal
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Cartilage Explant Culturespher the epistatic relationship between different signaling systems and other regulators of chondrocyte differentiation. Here we describe the protocol to culture cartilage explants ex vivo and discuss the advantages and disadvantages of the culture system.
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Methods in Molecular Biologyhttp://image.papertrans.cn/s/image/868189.jpg
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https://doi.org/10.1007/978-1-62703-989-5cell assays; skeletal development; skeletal repair; skeletal staining; skeletal tissue
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