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Titlebook: Skeletal Development and Repair; Methods and Protocol Matthew J. Hilton Book 2014 Springer Science+Business Media, LLC 2014 cell assays.ske

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书目名称Skeletal Development and Repair
副标题Methods and Protocol
编辑Matthew J. Hilton
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia
丛书名称Methods in Molecular Biology
图书封面Titlebook: Skeletal Development and Repair; Methods and Protocol Matthew J. Hilton Book 2014 Springer Science+Business Media, LLC 2014 cell assays.ske
描述.Skeletal Development and Repair: Methods and Protocols. is a compilation of a variety of skeletal research protocols utilizing the laboratory mouse as the platform for surgical manipulation and/or transplantation as well as the source of tissues and cells for .in vitro .culture and analyses. Chapters are written by experts in the field and cover topics including surgical, transplantation and organ culture methods that permit analyses of skeletal tissues undergoing repair .in vivo .and permits analyses of cellular interactions .ex vivo., histological and molecular techniques developed to study gene and protein expression in whole embryos, skeletal tissues and tissue sections and .in vitro .primary cell culture protocols designed to assay gene function in specific cell populations. Written in the successful .Methods in Molecular Biology. series format chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls..Authoritative and easily accessible, .Skeletal Development and Repair: Methods and Protocols. is a comprehensive laboratory manu
出版日期Book 2014
关键词cell assays; skeletal development; skeletal repair; skeletal staining; skeletal tissue
版次1
doihttps://doi.org/10.1007/978-1-62703-989-5
isbn_softcover978-1-4939-6310-2
isbn_ebook978-1-62703-989-5Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC 2014
The information of publication is updating

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Whole-Mount Skeletal Stainingnges in skeletal patterning. Because cartilage and bone can be distinguished by differential staining, this technique is also a powerful means to assess the pace of skeletal maturation. This protocol covers staining of the pre- and postnatal mouse skeleton using Alcian blue and Alizarin red to identify cartilage and bone, respectively.
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Nonradioactive In Situ Hybridization on Skeletal Tissue Sectionsanscript of interest. The labeled probe is then detected by immunohistochemical methods using an antibody conjugated to an enzyme that catalyzes the generation of chromogenic or fluorescent signals, which indicate the location of the transcript.
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