Titlebook: Single-Cell Protein Analysis; Methods and Protocol Aik T. Ooi Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive li

引起痛苦

Haphazard

Ultra-Sensitive Quantification of Protein and mRNA in Single Mammalian Cells with Digital PLA,ein and mRNA from the same single cell. The sensitivity of the assay has been further improved with implementation of the assay on a microfluidic system, which enables quantitation of rare protein species, with expression level as low as ~3000 protein molecules per cell.

群居动物

Generation of Centered Log-Ratio Normalized Antibody-Derived Tag Counts from Large Single-Cell Sequout the centered log-ratio (CLR) normalization of antibody-derived tag (ADT) counts that may be overlooked. In this method chapter, I provide detailed steps to generate CLR-normalized CITE-seq data using cloud computing from a large CITE-seq dataset.

和音

Comedienne

Combined Measurement of RNA and Protein Expression on a Single-Cell Level,odies conjugated with oligonucleotide (AbOs) for the surface marker detection, thus overcoming the limit of using few surface markers as occurs in flow cytometry. Here we describe the BD Rhapsody single-cell analysis system protocol for 3′ mRNA whole transcriptome analysis (WTA), combined with AbO- and Sample Tag library preparation.

开始没有

Immunochemistry Analysis Using Chromogenic Substrates on Tissue Sections,immunohistochemistry, it is possible to visualize the abundance, distribution, and localization of proteins in situ. This chapter discusses the standard protocols involved in IHC detection using chromogenic substrates, including pre-treatment of tissues, types of chromogenic substrates, and troubles

Charitable

Indirect Immunofluorescence of Tissue Sections,to achieve this understanding at the level of single cell granularity. Here, we describe the processes by which tissue is fixed, embedded, sectioned, and subsequently utilized for conducting indirect IF assays. We raise potential opportunities for troubleshooting and optimization at varying stages o

EVICT

Flow Cytometry for Beginners: Hints and Tips for Approaching the Very First Single-Cell Technique,protein analysis, as it allows to rapidly analyze and characterize protein expression in millions of cells..In this chapter, we will describe the main steps to prepare and acquire samples for flow cytometry, with particular focus on the setup of the right controls that are instrumental in analyzing

谈判

High-Dimensional Immunophenotyping with 37-Color Panel Using Full-Spectrum Cytometry,ective and practical way. Novel full-spectrum technology reveals the complete emission spectrum of each dye maximizing the amount of information that can be obtained on a single sample regarding conventional flow cytometry and provide an expanded knowledge of biological processes. In this chapter, w

恭维

Detection of Cytokine-Secreting Cells by Enzyme-Linked Immunospot (ELISpot),vel. The secreted molecules are detected by using a detection antibody system similar to that used in the enzyme-linked immunosorbent assay (ELISA). The ELISpot assay is carried out in a 96-well plate and an automated ELISpot reader is used for analysis. The assay is easy to perform, robust and allo