Titlebook: Single-Cell Protein Analysis; Methods and Protocol Aik T. Ooi Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive li

airborne

Combined Measurement of RNA and Protein Expression on a Single-Cell Level,ell isolation, based on surface marker expression predicted by the RNA expression profiles, is often limited by the poor correlation between transcript and protein expression patterns. To overcome these difficulties, novel single-cell multi-omic approaches based on the combined analysis of transcrip

生来

Simultaneous DNA, RNA, and Protein Analysis from Single Cells Using a High-Throughput Microfluidic origenesis and fostering progress in identifying druggable regimens for patient treatment scenarios. We report a new approach to measure multiple modalities simultaneously from up to 10,000 individual cells using microfluidics paired with next-generation sequencing. Our procedure determines targeted

敬礼

Immunochemistry Analysis Using Chromogenic Substrates on Tissue Sections,immunohistochemistry, it is possible to visualize the abundance, distribution, and localization of proteins in situ. This chapter discusses the standard protocols involved in IHC detection using chromogenic substrates, including pre-treatment of tissues, types of chromogenic substrates, and troubleshooting at various stages of the protocol.

飓风

细胞

充满装饰

Methods in Molecular Biologyhttp://image.papertrans.cn/s/image/867838.jpg

小卒

https://doi.org/10.1007/978-1-0716-1771-7Single-cell read outs; Protein expression data; Translational research; Signal amplification; Tissue sec

amyloid

adduction

舞蹈编排

Aik T. OoiIncludes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts