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Titlebook: Single-Cell Protein Analysis; Methods and Protocol Aik T. Ooi Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive li

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发表于 2025-3-21 19:53:44 | 显示全部楼层 |阅读模式
书目名称Single-Cell Protein Analysis
副标题Methods and Protocol
编辑Aik T. Ooi
视频video
概述Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Single-Cell Protein Analysis; Methods and Protocol Aik T. Ooi Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive li
描述This detailed volume serves as a collection of methods for single-cell protein analysis, created by combining different protocols, taking advantage of new emerging technologies, and improving upon conventional methods to guide researchers aiming to perform protein analysis in single cells. Ranging from simple to complex, conventional to the most current technologies, these chapters offer readers the ability to choose the best suited methodologies for them, based on the sample type and the available technologies or equipment. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Authoritative and practical, .Single-Cell Protein Analysis: Methods and Protocols. aims to help researchers utilizing single-cell protein analysis in their studies as well to inspire the development of the next generation of improved protein analysis methods in single cells..
出版日期Book 2022
关键词Single-cell read outs; Protein expression data; Translational research; Signal amplification; Tissue sec
版次1
doihttps://doi.org/10.1007/978-1-0716-1771-7
isbn_softcover978-1-0716-1773-1
isbn_ebook978-1-0716-1771-7Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightThe Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
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发表于 2025-3-21 23:15:00 | 显示全部楼层
Book 2022 new emerging technologies, and improving upon conventional methods to guide researchers aiming to perform protein analysis in single cells. Ranging from simple to complex, conventional to the most current technologies, these chapters offer readers the ability to choose the best suited methodologies
发表于 2025-3-22 01:34:50 | 显示全部楼层
Single Cell Proteomics Using Multiplexed Isobaric Labeling for Mass Spectrometric Analysis,e entire experimental design of single cell proteome analysis, exemplified by cultured A549 lung adenocarcinoma cells treated with an anti-cancer drug (methotrexate) and utilizing tandem mass tag (TMTpro™) labeling strategy for mass spectrometric data acquisition.
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发表于 2025-3-22 08:46:03 | 显示全部楼层
Simultaneous DNA, RNA, and Protein Analysis from Single Cells Using a High-Throughput Microfluidic protein levels, mRNA transcript levels, and somatic gDNA sequence variations including copy number variants. This approach can resolve over 20 proteins, 100s of targeted transcripts, and DNA amplicons.
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Detection of Cytokine-Secreting Cells by Enzyme-Linked Immunospot (ELISpot),he ELISpot assay is carried out in a 96-well plate and an automated ELISpot reader is used for analysis. The assay is easy to perform, robust and allows rapid analysis of a large number of samples and is not limited to measurement of cytokines; it is suitable for almost any secreted protein where single-cell analysis is of interest.
发表于 2025-3-23 08:35:10 | 显示全部楼层
Single-Cell Protein Profiling by Microdroplet Barcoding and Next-Generation Sequencing,n signals into DNA signals. Here, we describe how to prepare DNA-barcoded antibodies and connect protein identities to cellular identities using droplet microfluidics. This approach allows for multiplex single-cell protein analysis compatible with single-cell transcriptomic and mutational profiling methods.
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