Titlebook: Signal Transduction Protocols; David A. Kendall,Stephen J. Hill Book 19951st edition Springer Science+Business Media New York 1995

Glucocorticoids

改变立场

开始从未

The Measurement of Cyclic AMP Levels in Biological Preparations,eceptors. The mechanism of inhibition of adenylyl cyclase activity appears to be mediated through a distinct guanine nucleotide binding protein, G.. These two G-proteins act on adenylyl cyclase either to stimulate or inhibit the conversion of ATP to cAMP and PP..

Bravado

1064-3745 ists and pharmacologists, for instance, now need to embrace techniques that until recently were the strict preserves of biochemists and mole- lar biologists. However, the acquisition of new technologies can be a time-consuming and frustrating business, and unless an expert is on hand to give instruc

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Measurement of Phospholipase C Activity in Brain Membranes,rinic and 5HT.-sero-tonergic receptors in washed membrane preparations from brain. These assays differ basically from the more extended experimental designs involving [3.]inositol-labeled brain slices in the following aspects:

Insubordinate

LOPE

,Autoradiography of β1-and β2-Adrenoceptors,erive kinetic, saturation, competition binding, and receptor density data. This chapter describes methods for receptor autoradiography using specific ligands that can be used to localize, characterize, and quantitate ß.- and ß.-adrenoceptors in anatomically defined regions.

MIME

amphibian

ensemble

Mass Measurement of Key Phosphoinositide Cycle Intermediates,isphosphate [Ins(1,4,5)P.] and 1,2-diacylglycerol via inositol phospholipid-specific phospholipase C (PI-PLC) activity, and phosphatidylinositol 3,4,5-trisphosphate via phosphoinositide 3-kinase activity (.).