Titlebook: Signal Transduction Protocols; David A. Kendall,Stephen J. Hill Book 19951st edition Springer Science+Business Media New York 1995

词汇

The Problems and Pitfalls of Radioligand Binding,ied and solubilized receptors, membrane preparations, whole cells, tissue slices, and even whole animals. The basic method is very easy to perform. It can even be automated, and the throughput of samples that can be achieved is very high. The data obtained are typically extremely “tight” and reprodu

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Solubilization and Purification of a Functional Ionotropic Excitatory Amino Acid Receptor,e-spanning proteins that mediate the stimulatory actions of glutamate and possibly other related endogenous amino acids. Excitatory amino acid receptors are crucial for fast excitatory neurotransmission; they are believed to be involved in the neuropathology of ischemic damage; and they have been im

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LARK

malapropism

粗语

Arthropathy

The Measurement of Cyclic AMP Levels in Biological Preparations, by one of two routes. One group of receptors (such as β-adrenoceptors, A. adenosine receptors, D. dopamine receptors, histamine H. receptors, and some of the prostanoid receptors) is associated with an increased adenylyl cyclase activity and elevated cAMP levels mediated through a guanine nucleotid

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勋章

Cyclic AMP-Dependent Protein Kinase Activity Ratio Assay,evels and, in the vast majority of instances, the physiological effects are the result of activation of cAMP-dependent protein kinase (cA-PrK) and subsequent protein phosphorylation. The involvement of cA-PrK in the response may be investigated by the use of the “cA-PrK activity ratio” assay, first

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Purification and Activation of Cyclic GMP-Dependent Protein Kinase,ulfate precipitation, and cyclic nucleotide affinity chromatography (.). In this chapter, we describe the purification procedure that is routinely used in our laboratory. As a source, the bovine lung seems to be the best material to obtain a large amount of purified sample. However, the procedure de