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Titlebook: Signal Transduction Protocols; David A. Kendall,Stephen J. Hill Book 19951st edition Springer Science+Business Media New York 1995

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Cyclic AMP-Dependent Protein Kinase Activity Ratio Assay,evels and, in the vast majority of instances, the physiological effects are the result of activation of cAMP-dependent protein kinase (cA-PrK) and subsequent protein phosphorylation. The involvement of cA-PrK in the response may be investigated by the use of the “cA-PrK activity ratio” assay, first introduced in 1973 (.).
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The Analysis and Assay of Cyclic Nucleotide Phosphodiesterase Isoenzyme Activity,n as 3′,5′-cyclic nucleotide phosphodiesterases (PDEs; EC 3.1.4.17), which catalyze the hydrolytic cleavage of the 3′ phosphodiesterase bond to form the corresponding 5′ nucleotide (i.e., 5′AMP or 5′GMP; .).
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