Titlebook: Resin Microscopy and On-Section Immunocytochemistry; Geoffrey R. Newman,Jan A. Hobot Book 2001Latest edition Springer-Verlag Berlin Heidel

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Range-Of-Motion

Book 2001Latest editionation, as well as the great variety of labelling methods and markers that are commonly used for "on-section" cytochemistry and immunocytochemistry are described, including detailed protocols for the application.

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Handling Resin Blocksh a high meniscus. When warmed the reduction in surface tension is often strong enough to pull out wrinkles without recourse to organic solvents. Gelatin is digested by proteolytic enzymes and, therefore, should be avoided if section proteolysis for immunocytochemistry or . hybridisation is envisaged.

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Book 2001Latest edition the localisation of nucleic acid in tissue by in situ hybridisation. Resin-embedded tissue is routinely used and new techniques are constantly introduced. Thus, the novice entering these fields has a breathtaking variety of methods open to him. This labmanual covers the embedding of tissue using ep

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Cryotechniquesategies, Sect. 1.1). The prerequisite for cryotechniques is for biological tissue to be rapidly frozen prior to any processing steps. Processing can then be in to resin (Cryosubstitution, Sect. 4.3; Freeze-Drying, Sect. 4.4), or the frozen tissue can be sectioned directly (Cryoultramicrotomy, Sect. 4.5).

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Methods for Resin Polymerisationysts (Glauert 1975). However, considerably longer times are needed for the tissue to be present in an organic monomer. These methods are not used routinely and references to them in the literature are scarce.

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The Resins no such problems, were firmly established. The history and development of epoxy resins and the other group of beamstable, highly crosslinked resins, the polyesters, is well documented elsewhere (Glauert 1965, 1975, 1991).

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