| 书目名称 | Resin Microscopy and On-Section Immunocytochemistry | | 编辑 | Geoffrey R. Newman,Jan A. Hobot | | 视频video | | | 概述 | Ready-to-go protocols.Explains all steps from fixation to labelling and detection.Helps in finding the resin of choice for your needs | | 丛书名称 | Springer Lab Manuals | | 图书封面 |  | | 描述 | Since antibodies tagged with markers have been developed, immunocytochemistry has become the method of choice for identifying tissue substances or for the localisation of nucleic acid in tissue by in situ hybridisation. Resin-embedded tissue is routinely used and new techniques are constantly introduced. Thus, the novice entering these fields has a breathtaking variety of methods open to him. This labmanual covers the embedding of tissue using epoxy resin methods to the more sensitive procedures employing the acrylics. The possibilities and results are discussed so that an understanding of the techniques can be acquired and appropriate choices made. The various resins available and all steps involved in tissue processing, beginning with fixation, as well as the great variety of labelling methods and markers that are commonly used for "on-section" cytochemistry and immunocytochemistry are described, including detailed protocols for the application. | | 出版日期 | Book 2001Latest edition | | 关键词 | crytotechniques; electron microscopy; immunolabelling; resin embedding; tissue; tissue fixation | | 版次 | 2 | | doi | https://doi.org/10.1007/978-3-642-56930-2 | | isbn_softcover | 978-3-642-47731-7 | | isbn_ebook | 978-3-642-56930-2 | | copyright | Springer-Verlag Berlin Heidelberg 2001 |
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发表于 2025-3-21 16:25:19
