Titlebook: Resin Microscopy and On-Section Immunocytochemistry; Geoffrey R. Newman,Jan A. Hobot Book 2001Latest edition Springer-Verlag Berlin Heidel

涂掉

The Resinsrt et al. 1956; Glauert and Glauert 1958; Luft 1961; Glauert 1965, 1975). The epoxy resins gave uniform curing, and were found to be beamstable. Uncrosslinked methacrylate is only two-dimensionally bonded and is rapidly destroyed in the electron beam. It is also prone to shrinkage, sometimes uneven,

elastic

Cryotechniques sensitive to even low concentrations of fixative, or for preserving structures that cannot be stabilised during fixation or dehydration (Fixation Strategies, Sect. 1.1). The prerequisite for cryotechniques is for biological tissue to be rapidly frozen prior to any processing steps. Processing can t

Abjure

laceration

Handling Resin Blocksions can be picked up in the jaws of fine forceps and floated on to the surface of droplets of distilled water on glass-slides. They are dried down with gentle heat (< 60°C, 2–24 hours) and, without adhesive, will generally stick firmly to the surface of the glass. Problems with wrinkling or creasin

Isometric

剥皮

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Resin Embedding and Immunolabellingf these, other, more theoretical parameters were touched upon. A deeper understanding of such considerations may be important for “fine tuning” the sensitivity that can be obtained when labelling resin sections and for this reason they are more fully examined here.

苍白

TAP

armistice