Titlebook: Receptor Binding Techniques; Anthony P. Davenport Book 2012Latest edition Springer Science+Business Media, LLC 2012 G-protein-coupled rece

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Radioligand Binding Assays and Their Analysis,embrane-spanning G protein-coupled receptors for both established transmitters such as endothelin-1 and an increasing number of orphan receptors recently paired with their cognate ligands. Three types of assay are described. In saturation experiments, tissue sections, cultured cells, or homogenates

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Use of Scintillation Proximity Assay to Measure Radioligand Binding to Immobilized Receptors Withouee ligand in a receptor binding assay. SPA allows the rapid and sensitive assay of a wide range of molecular interactions in a homogeneous system and is routinely used for radioligand binding assays, particularly in drug screening applications where high throughput is required. Existing filter bindi

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Dissecting the Pharmacology of G Protein-Coupled Receptor Signaling Complexes Using Bimolecular Fluing partners such as G proteins or β-arrestins, or multimeric receptor complexes. Studying the ways in which such interactions modulate pharmacology requires techniques that report these events at the molecular level. Options include bimolecular fluorescence complementation (BiFC), an imaging-based

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,Measurements of β-Arrestin Recruitment to Activated Seven Transmembrane Receptors Using Enzyme Compupling to clathrin-mediated endocytosis. The nearly ubiquitous involvement of arrestin in 7TMR signaling has spurred the development of several methods for monitoring this interaction in mammalian cells. Nonetheless, few maintain the reproducibility and precision necessary for drug discovery applica

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Quantitative Phosphor Imaging Autoradiography of Radioligands for Positron Emission Tomography,r autoradiography. The major advantages of phosphor screens compared to radiation sensitive film are their greatly increased sensitivity, reducing exposure times with at least one order of magnitude, and their increased linear dynamic range. These features make phosphor screens ideal for imaging sho

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Cellular Localization of Receptors Using Antibodies Visualized by Light and Dual Labeling Confocal entification of cell types expressing a particular receptor when viewed using a conventional light microscope or by confocal microscopy. Protocols are also described for the dual labeling of cells in the same section using primary antisera raised in two different species (one to the receptor of inte