| 书目名称 | Receptor Binding Techniques |
| 编辑 | Anthony P. Davenport |
| 视频video | |
| 概述 | Updates the previous edition with cutting-edge content.Provides step-by-step detail essential for reproducible results.Contains key notes and implemenatation advice from the experts.Includes supplemen |
| 丛书名称 | Methods in Molecular Biology |
| 图书封面 |  |
| 描述 | .A broad definition of a receptor is a specialized protein on or in a cell that recognizes and binds a specific ligand to undergo a conformational change, leading to a physiological response or change in cell function. A ligand can be an endogenous neurotransmitter, hormone, paracrine/autocrine factor, or a synthetic drug that may function as an agonist or antagonist. The third edition of .Receptor Binding Techniques .expands upon the methods and techniques used for studying receptors in silico, in vitro and in vivo. Comprehensive chapters describe how to use online resources for experimental research such as prediction of receptor-ligand interactions and mine the IUPHAR receptor database. Classical techniques of radioligand binding, quantitative autoradiography and their analyses are complemented by the use of immunocytochemistry for the cellular localization of receptor protein and hybridization to detect receptor mRNA. Protocols using fluorescent labeled ligands are described to visualise receptors in living cells, their interaction with beta-arrestin to measure ligand-induced internalisation and green fluorescent protein to study trafficking. Non-radioactive, chemilumin |
| 出版日期 | Book 2012Latest edition |
| 关键词 | G-protein-coupled receptors; Immunocytochemistry; PET scanners; Radioligand binding; anatomical distribu |
| 版次 | 3 |
| doi | https://doi.org/10.1007/978-1-61779-909-9 |
| isbn_softcover | 978-1-4939-5936-5 |
| isbn_ebook | 978-1-61779-909-9Series ISSN 1064-3745 Series E-ISSN 1940-6029 |
| issn_series | 1064-3745 |
| copyright | Springer Science+Business Media, LLC 2012 |
发表于 2025-3-21 18:18:32
