Titlebook: Receptor Binding Techniques; Anthony P. Davenport Book 2012Latest edition Springer Science+Business Media, LLC 2012 G-protein-coupled rece

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Dissecting the Pharmacology of G Protein-Coupled Receptor Signaling Complexes Using Bimolecular Fluque, yet the simplicity of its fluorescent signal holds key advantages for quantification and cellular localization of the resultant complexes..This review introduces general experimental considerations for using the BiFC approach, and describes specific protocols to develop a BiFC assay for GPCR–β-

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,Measurements of β-Arrestin Recruitment to Activated Seven Transmembrane Receptors Using Enzyme Comp the enzyme, resulting in an enzymatic measure of the protein interaction. This chapter discusses the utility and methods involved in using the PathHunter β-galactosidase complementation system to monitor arrestin recruitment and the advantages of exploiting this pathway in the characterization of 7

汇总

Dynamic In Vivo Imaging of Receptors in Small Animals Using Positron Emission Tomography, imaging will significantly reduce the number of animals needed for this type of experiment as well as reducing the effect of inter-animal variation. Experimental protocols in small animal imaging potentially can be very labor intensive. In this chapter, we discuss methods and practical aspects rela

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Cellular Localization of Receptors Using Antibodies Visualized by Light and Dual Labeling Confocal yet known. The deletion of genes encoding receptors, particularly in mice, has emerged as a powerful tool in understanding the role of a specific receptor in physiological processes. Receptor immunocytochemistry can be used to analyze the resulting phenotype in whole body sections of mice without p

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Book 2012Latest editiond hybridization to detect receptor mRNA.  Protocols using fluorescent labeled ligands are described to visualise receptors in living cells, their interaction with beta-arrestin to measure ligand-induced internalisation and  green fluorescent protein to study  trafficking. Non-radioactive, chemilumin

松驰

Chidochangu P. Mpamhanga,Joanna L. Sharman,Anthony J. Harmar,NC-IUPHAR

盖他为秘密

Janet J. Maguire,Rhoda E. Kuc,Anthony P. Davenport

地名表

Daniel L. Bassoni,William J. Raab,Philip L. Achacoso,Chin Yee Loh,Tom S. Wehrman

ARK

Peter Johnström,Joseph L. Bird,Anthony P. Davenport

边缘

Peter Johnström,Tim D. Fryer,Joseph L. Bird,Hugh K. Richards,Anthony P. Davenport