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Titlebook: RNA Detection; Methods and Protocol Imre Gaspar Book 2018 Springer Science+Business Media LLC 2018 Drosophila.ECHO-liveFISH.FISH.RCas9.FISS

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书目名称RNA Detection
副标题Methods and Protocol
编辑Imre Gaspar
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: RNA Detection; Methods and Protocol Imre Gaspar Book 2018 Springer Science+Business Media LLC 2018 Drosophila.ECHO-liveFISH.FISH.RCas9.FISS
描述.This volume introduces different concepts and methods of detecting RNA in biological material in a variety of model systems. The chapters in this book discuss methods that will answer numerous biological questions that arise in the study of RNAs. Some of the topics covered in this book are single mRNA molecule detection in embryos and neurons.; .detection of mRNA and associated molecules by ISH-IEM on frozen sections; optimizing molecular beacons for intracellular analysis of RNA; imaging translation dynamics of single mRNA molecules in live cells; preparation of high-throughput sequencing libraries; and capturing RNA binding proteins in embryos and in cell-culture. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Cutting-edge and comprehensive, .RNA Detection: Methods and Protocols .is a valuable resource for novel and experiences scientist in the expanding field of RNAs..
出版日期Book 2018
关键词Drosophila; ECHO-liveFISH; FISH; RCas9; FISSEQ
版次1
doihttps://doi.org/10.1007/978-1-4939-7213-5
isbn_softcover978-1-4939-8418-3
isbn_ebook978-1-4939-7213-5Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media LLC 2018
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Toshiaki Shigeoka,Jane Jung,Christine E. Holt,Hosung Jungout ICT. As web technology has evolved from Web 1.0, consisting of simple computer systems, to Web 4.0, involving Artificial Intelligence and the Internet of Things, so has e-governance. As a result, there has been a noticeable transformation from a digital government to an algorithmic government. H
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