书目名称 | RNA Detection |
副标题 | Methods and Protocol |
编辑 | Imre Gaspar |
视频video | http://file.papertrans.cn/821/820157/820157.mp4 |
概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts |
丛书名称 | Methods in Molecular Biology |
图书封面 |  |
描述 | .This volume introduces different concepts and methods of detecting RNA in biological material in a variety of model systems. The chapters in this book discuss methods that will answer numerous biological questions that arise in the study of RNAs. Some of the topics covered in this book are single mRNA molecule detection in embryos and neurons.; .detection of mRNA and associated molecules by ISH-IEM on frozen sections; optimizing molecular beacons for intracellular analysis of RNA; imaging translation dynamics of single mRNA molecules in live cells; preparation of high-throughput sequencing libraries; and capturing RNA binding proteins in embryos and in cell-culture. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Cutting-edge and comprehensive, .RNA Detection: Methods and Protocols .is a valuable resource for novel and experiences scientist in the expanding field of RNAs.. |
出版日期 | Book 2018 |
关键词 | Drosophila; ECHO-liveFISH; FISH; RCas9; FISSEQ |
版次 | 1 |
doi | https://doi.org/10.1007/978-1-4939-7213-5 |
isbn_softcover | 978-1-4939-8418-3 |
isbn_ebook | 978-1-4939-7213-5Series ISSN 1064-3745 Series E-ISSN 1940-6029 |
issn_series | 1064-3745 |
copyright | Springer Science+Business Media LLC 2018 |